[HTML][HTML] Activation of c-Jun-NH2-kinase by UV irradiation is dependent on p21ras
V Adler, MR Pincus, A Polotskaya, X Montano… - Journal of Biological …, 1996 - ASBMB
We have demonstrated previously that Jun-NH 2-kinase (JNK) activation in vitro is
potentiated by association with the p21 ras protein. To determine if in vivo activation of JNK
also depends on p21 ras, we have used M1311 cells that carry the cDNA for the neutralizing
antibody to p21 ras, Y13-259, under a dexamethasone-inducible promoter. The ability of UV
to activate JNK gradually decreased over a 4-day period of cell growth in dexamethasone.
This decrease coincides with weaker transcriptional activation measured via gel shift and …
potentiated by association with the p21 ras protein. To determine if in vivo activation of JNK
also depends on p21 ras, we have used M1311 cells that carry the cDNA for the neutralizing
antibody to p21 ras, Y13-259, under a dexamethasone-inducible promoter. The ability of UV
to activate JNK gradually decreased over a 4-day period of cell growth in dexamethasone.
This decrease coincides with weaker transcriptional activation measured via gel shift and …
