[HTML][HTML] Sphingosine 1-phosphate is released from the cytosol of rat platelets in a carrier-mediated manner
N Kobayashi, T Nishi, T Hirata, A Kihara, T Sano… - Journal of lipid …, 2006 - ASBMB
Sphingosine 1-phosphate (S1P) is accumulated in platelets and released on stimulation by
thrombin or Ca 2+. Thrombin-stimulated S1P release was inhibited by staurosporin,
whereas Ca 2+-stimulated release was not. When the platelet plasma membrane was
permeabilized with streptolysin O (SLO), S1P leaked out with cytosol markers, whereas
granular markers remained in the platelets. The SLO-induced S1P leakage required BSA,
probably for solubilization of S1P in the medium. These results indicate that S1P is localized …
thrombin or Ca 2+. Thrombin-stimulated S1P release was inhibited by staurosporin,
whereas Ca 2+-stimulated release was not. When the platelet plasma membrane was
permeabilized with streptolysin O (SLO), S1P leaked out with cytosol markers, whereas
granular markers remained in the platelets. The SLO-induced S1P leakage required BSA,
probably for solubilization of S1P in the medium. These results indicate that S1P is localized …
