[HTML][HTML] A novel, in-solution separation of endogenous cardiac sarcomeric proteins and identification of distinct charged variants of regulatory light chain
SB Scruggs, R Reisdorph, ML Armstrong… - Molecular & Cellular …, 2010 - ASBMB
The molecular conformation of the cardiac myosin motor is modulated by intermolecular
interactions among the heavy chain, the light chains, myosin binding protein-C, and titin and
is governed by post-translational modifications (PTMs). In-gel digestion followed by
LC/MS/MS has classically been applied to identify cardiac sarcomeric PTMs; however, this
approach is limited by protein size, pI, and difficulties in peptide extraction. We report a
solution-based work flow for global separation of endogenous cardiac sarcomeric proteins …
interactions among the heavy chain, the light chains, myosin binding protein-C, and titin and
is governed by post-translational modifications (PTMs). In-gel digestion followed by
LC/MS/MS has classically been applied to identify cardiac sarcomeric PTMs; however, this
approach is limited by protein size, pI, and difficulties in peptide extraction. We report a
solution-based work flow for global separation of endogenous cardiac sarcomeric proteins …