[HTML][HTML] Familial hypertrophic cardiomyopathy mutations in the regulatory light chains of myosin affect their structure, Ca2+ binding, and phosphorylation
D Szczesna, D Ghosh, Q Li, AV Gomes… - Journal of Biological …, 2001 - ASBMB
The effect of the familial hypertrophic cardiomyopathy mutations, A13T, F18L, E22K, R58Q,
and P95A, found in the regulatory light chains of human cardiac myosin has been
investigated. The results demonstrate that E22K and R58Q, located in the immediate
extension of the helices flanking the regulatory light chain Ca 2+ binding site, had
dramatically altered Ca 2+ binding properties. The K Ca value for E22K was decreased by∼
17-fold compared with the wild-type light chain, and the R58Q mutant did not bind Ca 2+ …
and P95A, found in the regulatory light chains of human cardiac myosin has been
investigated. The results demonstrate that E22K and R58Q, located in the immediate
extension of the helices flanking the regulatory light chain Ca 2+ binding site, had
dramatically altered Ca 2+ binding properties. The K Ca value for E22K was decreased by∼
17-fold compared with the wild-type light chain, and the R58Q mutant did not bind Ca 2+ …