ELISA-quantitation of phosphorylated tau protein in the Alzheimer's disease brain
M Herrmann, S Golombowski, K Kräuchi, P Frey… - European …, 1999 - karger.com
M Herrmann, S Golombowski, K Kräuchi, P Frey, C Mourton-Gilles, C Hulette, C Rosenberg…
European neurology, 1999•karger.comA reliable, sensitive and specific sandwich ELISA for the quantitation of paired helical
filament (PHF) tau in human brain was developed using well-defined monoclonal
antibodies. We examined rapid-autopsy-derived brain tissue from 21 neuropathologically
confirmed Alzheimer's disease (AD) patients and 14 nondemented controls, matched for
age, sex and postmortem delay times. We demonstrated significant elevations of
phosphorylated tau levels in the frontal and parietal cortex as well as in the hippocampus of …
filament (PHF) tau in human brain was developed using well-defined monoclonal
antibodies. We examined rapid-autopsy-derived brain tissue from 21 neuropathologically
confirmed Alzheimer's disease (AD) patients and 14 nondemented controls, matched for
age, sex and postmortem delay times. We demonstrated significant elevations of
phosphorylated tau levels in the frontal and parietal cortex as well as in the hippocampus of …
Abstract
A reliable, sensitive and specific sandwich ELISA for the quantitation of paired helical filament (PHF) tau in human brain was developed using well-defined monoclonal antibodies. We examined rapid-autopsy-derived brain tissue from 21 neuropathologically confirmed Alzheimer’s disease (AD) patients and 14 nondemented controls, matched for age, sex and postmortem delay times. We demonstrated significant elevations of phosphorylated tau levels in the frontal and parietal cortex as well as in the hippocampus of AD patients as compared to the nondemented controls. No difference was observed in the cerebellum. Phosphorylated tau levels measured by ELISA were significantly correlated with the presence or absence of neurofibrillary tangles.
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