Positive regulation of hepatic miR-122 expression by HNF4α
BACKGROUND & AIMS: miR-122 is the most abundant microRNA in the liver and regulates
metabolic pathways including cholesterol biosynthesis, fatty acid synthesis, and oxidation.
However, little is known about mechanisms that regulate the expression of miR-122 in the
liver. The aim of this study was to identify key transcriptional regulators for miR-122
expression through intensively studying its primary transcript and promoter region.
METHODS: Bioinformatics analysis, Northern blotting, RT-PCR, and 5′/3′ RACE were …
metabolic pathways including cholesterol biosynthesis, fatty acid synthesis, and oxidation.
However, little is known about mechanisms that regulate the expression of miR-122 in the
liver. The aim of this study was to identify key transcriptional regulators for miR-122
expression through intensively studying its primary transcript and promoter region.
METHODS: Bioinformatics analysis, Northern blotting, RT-PCR, and 5′/3′ RACE were …
