[PDF][PDF] 5-Hydroxymethylcytosine marks sites of DNA damage and promotes genome stability
Cell reports, 2016•cell.com
hydroxymethylcytosine (5hmC) is a DNA base created during active DNA demethylation by
the recently discovered TET enzymes. 5hmC has essential roles in gene expression and
differentiation. Here, we demonstrate that 5hmC also localizes to sites of DNA damage and
repair. 5hmC accumulates at damage foci induced by aphidicolin and microirradiation and
colocalizes with major DNA damage response proteins 53BP1 and γH2AX, revealing 5hmC
as an epigenetic marker of DNA damage. Deficiency for the TET enzymes eliminates …
the recently discovered TET enzymes. 5hmC has essential roles in gene expression and
differentiation. Here, we demonstrate that 5hmC also localizes to sites of DNA damage and
repair. 5hmC accumulates at damage foci induced by aphidicolin and microirradiation and
colocalizes with major DNA damage response proteins 53BP1 and γH2AX, revealing 5hmC
as an epigenetic marker of DNA damage. Deficiency for the TET enzymes eliminates …
Summary
5-hydroxymethylcytosine (5hmC) is a DNA base created during active DNA demethylation by the recently discovered TET enzymes. 5hmC has essential roles in gene expression and differentiation. Here, we demonstrate that 5hmC also localizes to sites of DNA damage and repair. 5hmC accumulates at damage foci induced by aphidicolin and microirradiation and colocalizes with major DNA damage response proteins 53BP1 and γH2AX, revealing 5hmC as an epigenetic marker of DNA damage. Deficiency for the TET enzymes eliminates damage-induced 5hmC accumulation and elicits chromosome segregation defects in response to replication stress. Our results indicate that the TET enzymes and 5hmC play essential roles in ensuring genome integrity.
cell.com
